Checks: Lethality and deviations from normality
Variables: Coagulation, heartbeat, tail malformations and lack of somites
Delivery: LC50 is calculated based on the test
Checks: Movement patterns
Variables: Swim velocity, light-dark responses, circadian mobility rythms, sleep-wake parameters
Delivery: Detailed plot of circadian rythmicity, responses to light-dark stimuli and sleep-wake
parameters for each tested compound
Checks: Cardiovascular activity
Variables: Heart rate, rythmicity and blood circulation
Delivery: Detailed plot of dose response concentrations for each tested compound
Checks: Morphological variations
Variables: Head, tail, trunk, heart, eyes, circulation, fins and mobility
Delivery: LC50 and % of fish showing developmental defects
We have implemented a wide array of staining protocols using fluorescent and confocal imaging at our facilities, including TH staining for dopamine cells, and acH receptor staining at neuromuscular junctions.
With our expertise and equipment we can custom design a tox assay to address your specific research question at any time.