Our zebrafish-modelled sleep assay is one-of-a-kind.
It's the only one available today that allows researchers to gauge the sleep-inducing effects of drug molecules on in vivo subjects based on their behavioural phenotypes.
Zebrafish for sleep research
Since zebrafish exhibit a circadian rhythm and share other human CNS characteristics they can be effectively used in sleep research.
Our unique model tracks their behavioural patterns and analyses the effect of pharmaceutical molecules on their sleep cycles. All this is done on a not-seen-before scale, providing fast and surprisingly efficient results.
Behaviour is key
3Z Pharmaceuticals has built a verifiably unique process of evaluating drug compounds using zebrafish.
The key ingredient to our successful approach is our purpose-built analytics software that sheds a light on zebrafish behaviour. This gives researchers whole-organism data on an unprecedented scale.
Researching drug effects on sleep using zebrafish larvae
During our sleep assays we typically monitor 2304 zebrafish larvae at the same time.
8am - 10pm: Lights on
10pm - 8am: Lights off
Parameters extrapolated and analyzed
Average awakenings per hour
Percentage of time asleep during lights-off
Duration from lights-off to first sleep bout
Length of sleep bout
Average length of sleep bouts during lights-off
Length of wake bout
Average length of wake bouts during lights-off
Drug effects on sleep ratio
The 3Z Sleep Assay's large scale provides very clear results.
Here below you can see some of the results from a 1200-compound assay.
We plot the circadian rhythm observed for each compound being studied; at 3 different levels of concentration.
Drugs that exceed a certain cut-off point in sleep ratio are characterized as sleep-inducing and assayed again for confirmation.